(Linear) MAP of: w check: 72 from: 1 to: 429
With 270 enzymes: *
January 21, 2011 15:28 ..
CviKI-1
BlsI|
BisI||
Fnu4HI||
SetI GluI||
TaiI TseI|||
Hpy99I| BstNI ||||
HpyCH4IV || ScrFI |||| BmgT120I
Bme1580I | || BslI| |||| AvaII|
Bsp1286I | || BpuEI|| |||| Sau96I|
NlaIV | | || PspGI|| |||| BbvI ||
BanI | | | || StyD4I|| |||| SmlI | || AciI
| | | | || ||| |||| | | || |
ATGGGTGCCCCGACGTTGCCCCCTGCCTGGCAGCCCTTTCTCAAGGACCACCGCATCTCT
1 ---------+---------+---------+---------+---------+---------+ 60
TACCCACGGGGCTGCAACGGGGGACGGACCGTCGGGAAAGAGTTCCTGGTGGCGTAGAGA
a M G A P T L P P A W Q P F L K D H R I S -
b W V P R R C P L P G S P F S R T T A S L -
c G C P D V A P C L A A L S Q G P P H L Y -
TspDTI
BsaJI|
BtsCI|
CviKI-1||
FokI||
HaeIII||
EaeI |||
MnlI | |||
AciI | | |||
BsrBI | | |||
BslI| | | |||
BccI || | | |||
HpaII | || | | |||
NciI | || | | |||
ScrFI | || | | |||
BsaJI | | || | | |||
StyD4I | | || | | |||
HpyCH4V | | | || | | |||
Cac8I | | | | || | | |||
MwoI| | | | | || | | |||
HhaI|| | | | | || | | |||
GlaI||| | | | | || | | |||
BsgI HinP1I|||| | | | | || | | |||
MnlI | BlsI||||| | | | | || | | |||
BbvI | | BisI|||||| | | | | || | | |||
BsrI | | Fnu4HI|||||| | | | | || | | |||
BmgT120I| | | GluI|||||| | | | | || | | |||
CviKI-1| | | CviKI-1||||||| | | | | || | | |||
Hpy188III HaeIII| | | TseI||||||| | | | | || | | |||
SfaNI | Sau96I|| | | HpyAV |||||||| | | | | || | | |||
| | ||| | | | |||||||| | | | | || | | |||
ACATTCAAGAACTGGCCCTTCTTGGAGGGCTGCGCCTGCACCCCGGAGCGGATGGCCGAG
61 ---------+---------+---------+---------+---------+---------+ 120
TGTAAGTTCTTGACCGGGAAGAACCTCCCGACGCGGACGTGGGGCCTCGCCTACCGGCTC
a T F K N W P F L E G C A C T P E R M A E -
b H S R T G P S W R A A P A P R S G W P R -
c I Q E L A L L G G L R L H P G A D G R G -
CviKI-1
TspRI | BsrI
DdeI | | MboII
BtsCI NmeAIII | | | BmgT120I |
CviKI-1 | TspRI | | | | CviKI-1 |
Cac8I | | BspCNI | | | | | HaeIII |
FokI | |BseMII| | | | | | Sau96I| |
CviKI-1 | | |BtsI || | | | | | BmrI || | TspRI
| | | | | || | | | | | | || | |
GCTGGCTTCATCCACTGCCCCACTGAGAACGAGCCAGACTTGGCCCAGTGTTTCTTCTGC
121 ---------+---------+---------+---------+---------+---------+ 180
CGACCGAAGTAGGTGACGGGGTGACTCTTGCTCGGTCTGAACCGGGTCACAAAGAAGACG
a A G F I H C P T E N E P D L A Q C F F C -
b L A S S T A P L R T S Q T W P S V S S A -
c W L H P L P H * E R A R L G P V F L L L -
BseYI
CviKI-1
SetI |
AluI | |CviKI-1
CviKI-1 | | NlaIV|
HpyAV | | |GsaI || MnlI BslI BsmI
| | | | | || | | |
TTCAAGGAGCTGGAAGGCTGGGAGCCAGATGACGACCCCATAGAGGAACATAAAAAGCAT
181 ---------+---------+---------+---------+---------+---------+ 240
AAGTTCCTCGACCTTCCGACCCTCGGTCTACTGCTGGGGTATCTCCTTGTATTTTTCGTA
a F K E L E G W E P D D D P I E E H K K H -
b S R S W K A G S Q M T T P * R N I K S I -
c Q G A G R L G A R * R P H R G T * K A F -
ApoI
Tsp509I
MboII |
HhaI BsaJI| |
HpaII GlaI| MseI || |
BsaWI|HinP1I|| Hpy188III AjuI Tsp509I | StyI| |
|| ||| | | | | || |
TCGTCCGGTTGCGCTTTCCTTTCTGTCAAGAAGCAGTTTGAAGAATTAACCCTTGGTGAA
241 ---------+---------+---------+---------+---------+---------+ 300
AGCAGGCCAACGCGAAAGGAAAGACAGTTCTTCGTCAAACTTCTTAATTGGGAACCACTT
a S S G C A F L S V K K Q F E E L T L G E -
b R P V A L S F L S R S S L K N * P L V N -
c V R L R F P F C Q E A V * R I N P W * I -
AjuI HpyCH4V
HphI BsrI CviKI-1 Tsp509I |
| | | | |
TTTTTGAAACTGGACAGAGAAAGAGCCAAGAACAAAATTGCAAAGGAAACCAACAATAAG
301 ---------+---------+---------+---------+---------+---------+ 360
AAAAACTTTGACCTGTCTCTTTCTCGGTTCTTGTTTTAACGTTTCCTTTGGTTGTTATTC
a F L K L D R E R A K N K I A K E T N N K -
b F * N W T E K E P R T K L Q R K P T I R -
c F E T G Q R K S Q E Q N C K G N Q Q * E -
BbvI
CviAII
BsaJI|
BtgI|
FatI|
NcoI|
StyI|
BlsI ||
BisI| ||
Fnu4HI| ||
GluI| ||
CviKI-1|| ||
TseI|| ||
Cac8I ||| ||
SetI ||| ||
AluI | ||| ||
CviKI-1 | ||| ||
MspA1I | ||| ||
PvuII | ||| ||
BlsI| | ||| ||
BccI|| | ||| ||
BisI|| | ||| ||
Fnu4HI|| | ||| ||
GluI|| | ||| ||
ApoI HhaI TseI||| | ||| ||
Tsp509I AciI GlaI| TaqI |||| | ||| ||
MnlI|MboII BceAI | HinP1I|| BbvI |XcmI||| | ||| ||
|| | | | ||| | | |||| | ||| ||
AAGAAAGAATTTGAGGAAACTGCGGAGAAAGTGCGCCGTGCCATCGAGCAGCTGGCTGCC
361 ---------+---------+---------+---------+---------+---------+ 420
TTCTTTCTTAAACTCCTTTGACGCCTCTTTCACGCGGCACGGTAGCTCGTCGACCGACGG
a K K E F E E T A E K V R R A I E Q L A A -
b R K N L R K L R R K C A V P S S S W L P -
c E R I * G N C G E S A P C H R A A G C H -
NlaIII
|
ATGGATTGA
421 --------- 429
TACCTAACT
a M D * -
b W I -
c G L -
Enzymes that do cut:
AciI AjuI AluI ApoI AvaII BanI BbvI BccI
BceAI BisI BlsI Bme1580I BmgT120I BmrI BpuEI BsaJI
BsaWI BseMII BseYI BsgI BslI BsmI Bsp1286I BspCNI
BsrI BsrBI BstNI BtgI BtsI BtsCI Cac8I CviAII
CviKI-1 DdeI EaeI FatI Fnu4HI FokI GlaI GluI
GsaI HaeIII HhaI HinP1I HpaII HphI Hpy99I Hpy188III
HpyAV HpyCH4IV HpyCH4V MboII MnlI MseI MspA1I MwoI
NciI NcoI NlaIII NlaIV NmeAIII PspGI PvuII Sau96I
ScrFI SetI SfaNI SmlI StyI StyD4I TaiI TaqI
TseI Tsp509I TspDTI TspRI XcmI
Enzymes that do not cut:
AarI AatII AbsI AccI Acc65I AclI AcuI AfeI
AflII AflIII AgeI AhdI AleI AlfI AloI AlwI
AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanII BarI BbeI BbsI BbvCI BcgI
BciVI BclI BdaI BfaI BglI BglII BlpI BmeT110I
BmgBI BmtI BplI BpmI Bpu10I BsaI BsaAI BsaBI
BsaHI BsaXI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstKTI BstUI BstXI BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI CviQI DpnI DraI
DraIII DrdI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FauI FseI FspI FspAI
HaeII HgaI Hin4I HincII HindIII HinfI HpaI Hpy166II
Hpy188I HpyCH4III KasI KpnI MaeIII MauBI MboI MfeI
MluI MlyI MmeI MreI MscI MslI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PleI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsaI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgrAI SgrDI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TatI TauI TfiI TsoI Tsp45I TspGWI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
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